Composite

Part:BBa_K5048018:Design

Designed by: Yecheng Zhao   Group: iGEM24_WHU-China   (2024-09-13)


pBAD-Lpp'OmpA-DDDDK-AQ-GST-dt


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 239
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 74
    Illegal AgeI site found at 655
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 56


Design Notes

We replace the T7 promoter with araBAD promoter to prevent the leakage of promoter



Source

complete synthesis

References